Figure 7.

Analyses of dysregulated signaling pathways in cardiomyocyte hypertrophy regulated by micropeptides

(A–C) Heatmaps showing the expression of dys-regulated genes in significantly altered KEGG pathways in Ad-sORF8 (A)-, Ad-sORF7 (B)-, and Ad-sORF6 (C)-overexpressed NRVCs under the treatment of PE. (D–F) Selected gene expression was confirmed with qRT-PCR in Ad-sORF8 (D)-, Ad-sORF7 (E)-, and Ad-sORF6 (F)-overexpressed NRVCs under the treatment of PE. (G and H) Detection of the expression of phosphorylated and total ERK in Ad-sORF6 (G)- and Ad-sORF7 (H)-overexpressed NRVCs with or without the treatment of PE by western blotting. The ratio of phospho-ERK to total ERK was quantified. Data were obtained from biological replicates from six independent experiments (n = 6) for each group. ∗p < 0.05, ∗∗p < 0.01.