Figure 6.

SAS treatment of melanoma leads to M2 macrophage polarization

(A) A schematic of the conditioned medium collection protocol is shown. (B and C) mRNA was extracted from conditioned medium-treated macrophages as indicated, and quantitative real-time PCR was then performed. (D) A schematic of the coculture plan is shown. (E) SAS-treated melanoma cells were cocultured with macrophages as indicated, and the proportions of F4/80⁺CD11B⁺CD206⁺ macrophages were analyzed by FACS. (F) Cell lysates were extracted from conditioned medium-treated macrophages as indicated, and immunoblotting was then performed. Data from multiple experiments are expressed as the mean (n = 3) ± SD. Asterisks indicate significant differences: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.