Figure 2.

Preparation and internalization of AEs

(A) let-7a and let-7b are among the top common miRNAs in all angiogenic EVs tested. (B) miRNA-loaded AEs with the size of ∼50 nm can be prepared by microfluidic micromixing. Scale bar is 200 nm in TEM picture. (C) Flow cytometry analysis of Cy5-cel-miR-39-AEs internalized by HUVECs under hypoxia (1% O₂). (D and E) AEs can pass their cargo (cel-miR-39 in the graphs) to HUVECs under both hypoxia (1% O₂) and normoxia (20% O₂). Relative expression was given as 2^−ΔΔCt (normalization to untreated cell group). In all graphs, values are given as average ± SEM (n = 2−4).