Figure 4.

Fibrin gel bead assay to evaluate the angiogenic potential of let-7b-AEs in HUVECs under hypoxic conditions (1% O₂)

(A and B) Representative images (A) and quantification of sprout length and number of sprouts (n = 4) (B) after 30 h. 30 nM let-7b-AEs were used in the experiments. White arrows indicate the sprouts. In all graphs, values are given as average ± SEM (n = 5−10). ∗∗p ≤ 0.01. Scale bars, 300 μm. Comparison across subgroups was performed with Kruskal-Wallis test.