Figure 5.

TMEM229A inhibits NSCLC progression partly via the ERK signaling pathway. (A) Western blotting examination of p-ERK, E-cadherin, N-cadherin, Snail1 and MMP2 expression after TMEM229A knockdown with or without PD98059 treatment (ERK inhibitor; 20 µM). The ratios of p-AKT (Ser473)/t-AKT and p-ERK/t-ERK were analyzed. (B) Cell invasion was measured using a Transwell assay in H1299 cells after knockdown of TMEM229A with or without PD98059 treatment (ERK inhibitor; 20 µM). *P<0.05, **P<0.01 and ***P<0.001. TMEM229A, transmembrane protein 229A; NSCLC, non-small cell lung cancer; si-Ctrl, scrambled siRNA; p-, phosphorylated; t-, total; Snail1, snail family transcriptional repressor 1; si-, small interfering.