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. 2021 Jun 23;12:689410. doi: 10.3389/fimmu.2021.689410

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Copyright © 2021 Amponnawarat, Chompunud Na Ayudhya and Ali

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Naturally occurring MRGPRX2 missense variants are hypo-responsive to murepavadin for MC degranulation. (A) Snake diagram of MRGPRX2 indicating the two amino acid residues to be investigated. (B) Single amino acid substitution for each of the MRGPRX2 variant is shown in the table. (C) Cell surface receptor expression of the wild-type MRGPRX2 (WT) and its missense variants (G165E and V282M) was confirmed using flow cytometry. (D) RBL cells expressing MRGPRX2 (WT) and its variants (G165E and V282M) were exposed to 10 μM murepavadin (30 min) and degranulation was assayed by measuring the release of β-hexosaminidase. Statistical significance was determined by two-way ANOVA with Tukey’s multiple comparisons at a value **p < 0.01 and ****p < 0.0001.