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. 2021 Jun 23;12:694720. doi: 10.3389/fimmu.2021.694720

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Copyright © 2021 Jin, Shen, Pan, Zhu, Li, Xu, Betancor, Jiao, Tocher and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Paraffin section of liver of juvenile black seabream (Acanthopagrus schlegelii) fed the experimental diets (Control: 11% crude lipid; HFD: high-fat diet; HFD+B1: HFD +betaine (10g/kg); HFD+B2: HFD + betaine (20 g/kg)) for 8 weeks. The liver sections were stained with H&E to enhance the contrast (400X). (A) Paraffin section of liver of Control group; (B) Paraffin section of liver of HFD group; (C) Paraffin section of liver of HFD+B1 group; (D) Paraffin section of liver of HFD+B2 group. F, Fat drop; C, Cell nucleus.