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. 2021 Jun 23;12:694720. doi: 10.3389/fimmu.2021.694720

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Copyright © 2021 Jin, Shen, Pan, Zhu, Li, Xu, Betancor, Jiao, Tocher and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inflammation gene expression in liver and intestine of juvenile black seabream (Acanthopagrus schlegelii) (% wet weight) fed the experimental diets [Control: 11% crude lipid; HFD: high-fat diet; HFD+B1: HFD +betaine (10g/kg); HFD+B2: HFD + betaine (20 g/kg)] for 8 weeks. (A) The relative mRNA expression of tgfβ-1, il-10, nfκb, tnf-α and il-1β in liver. (B) The relative mRNA expression of tgfβ-1, il-10, nfκb, tnf-α and il-1β in intestine. Control was used as the reference group, and the mRNA expression levels of target genes were normalized relative to the expression of β-actin. Values are means (n = 3) with standard errors represented by vertical bars. Mean values for the same gene with different letters were significantly different (P < 0.05).