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. 2021 Jun;12(3):953–963. doi: 10.21037/jgo-21-258

Figure 1.

Figure 1

YAP depletion attenuates CRC cell proliferation, migration, and invasion and increases cell apoptosis in vitro. (A) In multiple CRC cell lines, YAP expression levels were detected by immunoblot using YAP-specific antibodies, and the results showed that high YAP levels were observed in BRAF V600E mutated CRC cells. (B,C) RKO was transfected with YAP plasmid for 24 hours, HT29 was transfected with YAP-shRNA virus for 24 hours, and fluorescence microscopy was used to verify the transfection results. YAP levels were then tested by Western blot analysis. (D) RKO was transfected with YAP plasmid and HT29 was transfected with yap-shrna virus for 24,48, and 72 hours. Cell viability was determined by MTS method. (E) Colony formation assays, in which 500 HT 29, or RKO cells were treated with shYAP for 7 days. Representative images. (F) The wound healing assay. Wound fields were photographed at 0, 24, and 48 h after wounding at three different sites. Representative images. (G,H) Transwell invasion assay was performed. Representative images of the invasive cells on the membrane. CRC, colorectal cancer; YAP, yes-associated protein. **P<0.05, ***P<0.01. ****P<0.001.