Figure 3.

BRAF, MEK inhibitors, and YAP have a synergistic effect in regulating the cell cycle, increasing G1/S phase arrest. (A) Through flow cytometry to detect the cell cycle, we found that YAP knockdown can synergistically increase the G1/S phase block in the cell cycle of RKO and HT29CRC cells treated with RAF→MEK pathway inhibitors. (B) The nuclear expression of YAP in RKO and HT29CRC cells was observed in Western Blot. (C,D) WB confirmed that CDK6 was downregulated in both mRNA and protein levels after YAP knockdown. (E) BRAF and MEK inhibitors have synergistic effects with YAP in regulating cell cycle, increasing G1/S phase arrest. YAP, yes-associated protein; MEK, MAPK/extracellular signal-regulated kinase.