. 2021 Jun 29;15:262–269. doi: 10.1016/j.ijppaw.2021.06.006

Table 2.

Conserved primers and PCR amplification conditions used in this study to amplify the 18S rRNA gene of Babesia, Theileria, Trypanosoma and Sarcocystis, as well as the Plasmodium cytochrome b gene.

Parasite genus	Gene	Primer name	Primer sequence (5’ – 3′)	Amplicon	PCR conditions
Parasite genus	Gene	Primer name	Primer sequence (5’ – 3′)	Amplicon	annealing	extension
*Babesia* *Theileria*	18 S rRNA	Piro1-S	CTTGACGGTAGGGTATTGGC	1400 bp	55 °C	90 s
*Babesia* *Theileria*	18 S rRNA	Piro3-AS	CCTTCCTTTAAGTGATAAGGTTCAC	1400 bp	55 °C	90 s
*Trypanosoma*	18 S rRNA	S825F	ACCGTTTCGGCTTTTGTTGG	950 bp	60 °C	60 s
*Trypanosoma*	18 S rRNA	SLIR	ACATTGTAGTGCGCGTGTC	950 bp	60 °C	60 s
*Sarcocystis*	18 S rRNA	cocc18SF	GAAAGTTAGGGGCTCGAAGA	400 bp	57 °C	45 s
*Sarcocystis*	18 S rRNA	cocc18SR	CCCTCTAAGAAGTGATACA	400 bp	57 °C	45 s
*Plasmodium*	cytochrome b	3932 F	GGGTTATGTATTACCTTGGGGTC	750 bp	57 °C	60 s
*Plasmodium*	cytochrome b	DW4	TGTTTGCTTGGGAGCTGTAATCATAATGTG	750 bp	57 °C	60 s