Figure 1.

Poor survival and squamous cell carcinoma in Arid1a^flox/flox; Kras^{Lox-Stop-Lox-G12D/+}; Pgr^Cre/+ (AKP) female mice. A: Kaplan-Meier survival curves were analyzed by the log-rank (Mantel-Cox) test. AKP mice had decreased survival compared with control mice. B: Gross vaginal tumor burden (white arrow) in AKP mice. C: Gross lesions were localized to the vagina with grossly normal ovaries, oviducts, uterus, and cervix. Tick marks on ruler indicate 1 mm. D: Control vagina had normal keratinized stratified squamous epithelium (SSE) and lamina propria (LP). E: AKP vagina exhibited squamous cell carcinoma lined by highly dysplastic squamous epithelium (DSE) with central keratinization (star) and nests of cells (dashed circle) with abundant eosinophilic cytoplasm (black arrow). Hematoxylin and eosin staining. n = 10 per group (A). ∗∗∗P < 0.001. Scale bars: 50 μm (D and E, main images); 200 μm (D and E, insets).