Figure 2.

Squamous intraepithelial lesions and squamous cell carcinoma in Arid1a^flox/flox; Kras^{Lox-Stop-Lox-G12D/+}; Pgr^Cre/+ (AKP) mice. A: Schematic timeline of the progression of squamous intraepithelial lesions to squamous cell carcinoma in AKP mice. B: At 8 weeks, Pgr^+/+ vagina had normal stratified squamous epithelium (SSE). C: Ten percent of AKP vaginas contained low-grade squamous intraepithelial lesions (LSILs) with increased nuclear/cytoplasmic ratios and loss of maturation (LOM) in the lower epithelium. D: Seventy percent of AKP vaginas exhibited high-grade squamous intraepithelial lesions (HSILs) with cell crowding, high nuclear/cytoplasmic ratio, and LOM in at least two-thirds of the epithelium. E: Twenty percent of AKP vaginas had squamous cell carcinoma with nests of cells (dashed circle) and highly dysplastic squamous epithelium invading through the stroma (black arrow). Hematoxylin and eosin staining. Scale bar = 20 μm.