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. 2021 Jun 1;13(7):e12872. doi: 10.15252/emmm.202012872

Figure EV2. miR‐9 positively regulates Sp1 expression.

Figure EV2

  1. qRT–PCR analyses of Sp1 expression in control (shCTR) and anti‐miR‐9 (miR‐9 sponge) FaDu cells. Data represent the mean (± SD) of three independent experiments performed in duplicate, and unpaired t‐test was used to verify the statistical significance.
  2. qRT–PCR analyses of Sp1 expression in CAL27 cells transiently transduced with and PGK‐miR‐9 (GFP‐miR‐9) or control (GFP control) vector as indicated. Data represent the mean (± SD) of three independent experiments performed in duplicate, and unpaired t‐test was used to verify the statistical significance.
  3. WB analyses of the indicated protein expression in control (shCTR) and miR‐9 CAL27 cells, overexpressing or not RSV‐Sp1 as indicated. Actin was used as loading control.
  4. Graphs reporting the cell viability of CAL27 cells described in (C) and treated with increasing concentration of gefitinib (GEFI) and evaluated using the MTS assay. Data represent the mean (± SD) of two independent experiments performed in sextuplicate, and two‐way ANOVA with Sidak’s multiple comparison test was used to verify the statistical significance.
  5. Graph reporting the normalized luciferase activity of Sp1 promoter fragments in CAL27 cells control or stably overexpressing miR‐9. Data are the mean (± SD) of three independent experiments performed in duplicate, and two‐way ANOVA with Sidak’s multiple comparison test was used to verify the statistical significance.

Data information: In the figure, A.U. = arbitrary units; *P < 0.05; **P < 0.01; ***P < 0.001.

Source data are available online for this figure.