Skip to main content
. 2021 Jun 1;13(7):e12872. doi: 10.15252/emmm.202012872

Figure EV4. miR‐9 protects HNSCC cells from RT‐induced cell death.

Figure EV4

  1. Graph reporting cell viability of control (shCTR) and anti‐miR‐9 SCC9 cells treated with increasing concentration of the indicated drugs for 72 h and analyzed using the MTS cell viability assay. Data represent the mean (± SD) of three independent experiments each performed in sextuplicate. Unpaired t‐test was used to calculate the statistical significance at each dose.
  2. Clonogenic assay of control (shCTR) and anti‐miR‐9 SCC9 cells not irradiated (NIR) or treated with 2 or 5 Gy IR. On the left, typical images of cell clones are shown, and on the right, the graph reports the percentage (± SD) of survived cells respect to not irradiate cells in three independent experiments performed in triplicate. Unpaired t‐test was used to calculate the statistical significance at each dose.
  3. Left, WB analyses of the indicated protein expression in control (shCTR) and anti‐miR‐9 SCC9 cells not irradiated (NIR) or treated with 2 Gy IR and allowed to repair for the indicated hours (h). Tubulin was used as loading control. On the left, graphs report the quantification of the indicated proteins normalized on tubulin expression. Data are expressed as mean (± SD) of three independent experiments.

Data information: In the figure, A.U. = arbitrary units; unpaired t‐test was used to calculate the statistical significance at time point.*P < 0.05; **P < 0.01; ***P < 0.001.

Source data are available online for this figure.