Figure 7.

Depletion of Plk1 and inhibition of DNMT3a suppress DU145‐derived xenograft tumor growth synergistically. A) Nude mice were inoculated with DU145 cells depleted of Plk1 (2.5 × 10⁵) with lentivirus and treated with 5‐Aza (3.3 mg kg⁻¹, intraperitoneal injection), followed by measurement of tumor size every week (means ± SE). Tumor growth for different groups was analyzed by Two‐way ANOVA test. B) Body weight was measured at the end of the study. C) Tumor images at the end of the study. D) Wet weights of tumors. E) IHC staining for Ki67, cleaved caspase 3, Plk1, DNMT3a, and p‐DNMT3a in xenograft tumors. F) Quantification of (E). G) Quantification of RBC and WBC in mice received treatments. n = 4 for sh‐ctl and sh‐Plk1 groups, n = 5 for sh‐ctl+5‐Aza and sh‐Plk1+5‐Aza groups. Tumor mass data were analyzed by unpaired Student t‐test. *p<0.05, **p<0.01, ***p<0.001.