TABLE 1.
Abbreviations and phenotype information of pig sorted immune cells.
| Gatea | Population Abbreviation | Marker | Clone | Fluorophore | Company (Catalog #)b | FACS Sort Criteria |
| CD3ε + MACS Fraction | ||||||
| − | − | Anti mouse IgG1 | RMG1-1 | PE-Cy7 | BioLegend(406614) | − |
| 1 | SWC6gdT | SWC6gdT | MAC320 | APC | BD(561482) | CD3ε+SWC6+ |
| 2 | CD4T | CD4 | 74-12-4 | FITC | BD(559585) | CD3ε+SWC6–CD4+CD8α– |
| 3 | CD4CD8T | CD4CD8α | 74-12-4/76-2-11 | FITC/PE | BD(559585)/BD(559584) | CD3ε+SWC6–CD4+CD8α+ |
| 4 | CD8T | CD8α | 76-2-11 | PE | BD(559584) | CD3ε+SWC6–CD4–CD8α+ |
| CD3ε – MACS Fraction | ||||||
| 5 | Myeloid | CD172 | 74-22-15A | FITC | BD(561498) | CD3ε–CD172α+CD8α– |
| 6 | NK | CD8α | 76-2-11 | PE | BD(559584) | CD3ε–CD172α–CD8α+ |
| 7 | CD21pB | CD21 | BB6-11C9.6 | AF647 | Southern Biotech(4530-31) | CD3ε–CD172α–CD8α–CD21+ |
| 8 | CD21nB | − | − | − | − | CD3ε–CD172α–CD8α–CD21– |
aRefers to gate in Figure 1. bReagents listed in Materials and Methods.