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. 2021 Jun;10(6):1658–1667. doi: 10.21037/tp-21-58

Figure 2.

Figure 2

Novel approaches developed for the screening of the mutations c.852_855del and c.1751-5_1751-4ins(2684). (A) On c.852_855del screening, the wild-type SLC25A13 genotype exhibited 2 bands of 583 bp and 969 bp in size, the homozygote showed 2 bands of 445 bp and 965 bp, while the heterozygote showed all 4 bands. In this figure, the 2 bands of 965 bp and 969 bp in sizes represent the PCR products with and without the mutation c.852_855del, respectively. (B) PCR products of the wild-type and homozygote of c.1751-5_1751-4ins(2684) only exhibited a band of 335 bp and 564 bp, respectively, while that of the heterozygote presented with both bands. (C) Sanger sequencing of PCR products of c.852_855del. (D) Sanger sequencing of PCR products of c.1751-5_1751-4ins(2684).