Table 1.

List of primers.

RT-PCR Nrxns
Nrxns SS4 splice variants [43]
Nrxn1 (+/−SS4)	Fw: 5′-TGT TGG GAC AGA TGA CAT CGC C-3′
	Rv: 5′-GAG AGC TGG CCC TGG AAG GG-3′
Nrxn2 (+/−SS4)	Fw: 5′-GTG CGC TTT ACT CGA AGT GGT G-3′
	Rv: 5′-CCC ATT GTA GTA GAG GCC GGA C-3′
Nrxn3 (+/−SS4)	Fw: 5′-TTG TGC GCT TCA CCA GGA ATG-3′
	Rv: 5′-AGA GCC CAG AGA GTT GAC CTT G-3′
Nrxns Alpha/Beta [44]
Nrxn1 (+/−SS4)	Fw(α): 5′-CAG CAC AAC CTG CCA AGA-3′
	Fw(β): 5′-CCT GGC CCT GAT CTG GAT AGT-3′
	Rv(αβ): 5′-GAG AGC TGG CCC TGG AAG GG-3′
Nrxn2 (+/−SS4)	Fw(α): 5′-CAC CAC CTG CAC CGA AGA G-3′
	Fw(β): 5′-GTG CCC ATC GCC ATC AA-3′
	Rv(αβ): 5′-CCC ATT GTA GTA GAG GCC GGA C-3′
Nrxn3 (+/−SS4)	Fw(α): 5′-CTG TGA CTGCTC CAT GAC ATC ATATT-3′
	Fw(β): 5′-AAGCACCACTCTGTGCCTATTTCT-3′
	Rv(αβ): 5′-AGA GCC CAG AGA GTT GAC CTT G-3′
RT-qPCR Nrxns
Nrxns +/−SS4 splice variants:
Nrxn1 (+SS4)	Fw: 5′-TAG TTG ATG AAT GGC TAC TCG ACA AA-3′
	Rv: 5′-GAC TCA GTT GTC ATA GAG GAA GGC AC-3′
Nrxn1 (−SS4)	Fw: 5′-GCT ACC CTG CAG GGC GT-3′
	Rv: 5′-GAG GTG GAC ATC TCA GAC TGC AT-3′
Nrxn2 (+SS4)	Fw: 5′-AAT CCC CTA CCG GCT TGG T-3′
	Rv: 5′-CCC CCT ATC TTG ATG GCA GC-3′
Nrxn2 (−SS4)	Fw: 5′-AGA GGT ACC CGG CAG GAC-3′
	Rv: 5′-GAC ACC TGG CCC TGG AAG-3′
Nrxn3 (+SS4)	Fw: 5′-AGG AGT GGC TGC AGG AAA AA-3′
	Rv: 5′-TTG TCC TTT CCT CCG ATG GC-3′
Nrxn3 (−SS4)	Fw: 5′-GAG CAC TAT CCT ACA GGC CG-3′
	Rv: 5′-AGA GTT GAC CTT GGA AGA GAC G-3′
Nrxns constant region (RC)
Nrxn1 (RC)	Fw: 5′-CTGGAGCTGCACATACACCA-3′
	Rv: 5′-TGCCACCACTCCTTGTGAAA-3′
Nrxn2 (RC)	Fw: 5′-GTAGGCTTCAGCACACACCA-3′
	Rv: 5′-TCGTCCGTGCCCACATTAAA-3′
Nrxn3 (RC)	Fw: 5′-CTGGACTTGGCGACTTCCTC-3′
	Rv: 5′-TGAAGCGCACAACGTGGTAT-3′
β-Actin	Fw: 5′-CTG TCG AGT CGC GTC CAC-3′
	Rv: 5′-GCT TTG CAC ATG CCG GAG-3′
RT-qPCR
Cdc42	Fw: 5′-TGCTCTGCCCTCACACAGAAAG-3′
	Rv: 5′-GCGGCTCTTCTTCGGTTCTG-3′
Robo2	Fw: 5′-CGAGCTCCTCCACAGTTTGT-3′
	Rv: 5′-GTAGGTTCTGGCTGCCTTCT-3′
Shank3	Fw: 5′-GCCTTCCCTGCACTCCAATA-3′
	Rv: 5′-GCTTGTGTCCAACCTTCACG-3′
Nestin	Fw: 5′-GCAGGAGAAGCAGGGTCTAC-3′
	Rv: 5′-GAGTTCTCAGCCTCCAGCAG-3′
Mhc I	Fw: 5′-AGGGCGACCTCAACGAGAT-3′
	Rv: 5′-CAGCAGACTCTGGAGGCTCTT-3′
Mhc IIa	Fw: 5′-CCAAGAAAGGTGCCAAGAAG-3′
	Rv: 5′-CGGGAGTCTTGGTTTCATTG-3′
Mhc IIb	Fw: 5′-GCTTGAAAACGAGGTGGAAA-3′
	Rv: 5′-CCTCCTCAGCCTGTCTCTTG-3′
Mhc IIx	Fw: 5′-CGGTGGTGGAAAGAAAGG-3′
	Rv: 5′-CAGGAGTCTTGGTTTCATT-3′
CypA	Fw: 5′-GTCAACCCCACCGTGTTCTT-3′
	Rv: 5′-CTGCTGTCTTTGGGACCTTGT-3′