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. 2021 Jun 17;24(7):102739. doi: 10.1016/j.isci.2021.102739

Figure 4.

Figure 4

MRTF and SRF localize to the basal body and the primary cilium

(A) LLC-PK1 (left panels) and RPE cells (right panels) were serum-deprived and co-stained for MRTF and the basal body marker γ-tubulin. Staining was visualized by confocal microscopy. Scale bar 20 μm and 5 μm for full and boxed images, respectively. The last panels (Box) show the magnified regions demarcated by the dashed rectangles in the corresponding merged images. Note that distinct MRTF patches colocalize or partially overlap with γ-tubulin (BB), but MRTF staining occasionally penetrates the cilium as well.

(B) LLC-PK1 cells (left panels) and RPE cells (right panels) were co-stained for SRF and the cilium marker acetylated tubulin. The last panels (Box) show the magnified regions demarcated by the dashed rectangles in the corresponding merged images. Note that SRF, as expected, resides primarily in the nucleus. In addition, SRF also shows clear localization to the primary cilium in a subset of cells.