Fig. 6.
Effect of hepatic Surf4 knockout on gene expression. Mice were treated as described in the legend to Fig. 3. A–E: Quantitative real-time PCR (six mice per group). The relative mRNA levels were the ratio of the mRNA level of the target to that of Gapdh at the same condition. F: Western blot of liver homogenate with antibodies indicated. The relative densitometry was the ratio of the densitometry of the protein indicated to that of actin (n = 6). Representative images were shown. G: Plasma ketone bodies were measured using a kit (Nanjing Jiancheng Bioengineering Institute) (n = 7). H: FA oxidation. Primary hepatocytes isolated from Surf4Flox or Surf4LKO mice were subjected to measurement of FA oxidation using a commercial kit from abcam. Student's t-test was used to determine the significant differences between groups. Values of all data were mean ± SD. The significance was defined as ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001. P > 0.05, no significant difference (ns).