Fig. 7.

The effect of Surf4 knockdown on plasma lipid levels and the expression of apoB and apoA-I in Ldlr^−/− mice. About 8–10-week-old male Ldlr^−/− mice were injected with AAV-scrambled (Scram.) or Surf4 shRNA and then fed the Western-type diet for 14 weeks. A: Quantitative real-time PCR. The relative mRNA levels were the ratio of the mRNA levels of the target gene to that of Gapdh (n = 6). B: TG secretion. Blood was collected from mice before and 0.5, 1, 2, and 4 h after P-407 injection. Plasma TG was measured using a kit (Wako Diagnostics) (n = 4). C and D: Immunoblotting of the same amount of plasma (C) or liver homogenate (D) with antibodies indicated (n = 6). Representative images were shown. E–H: Plasma lipids were measured using kits (Wako Diagnostics) (n = 6). I and J: Lipid profile. About 5 μl of plasma from each mouse in the same group (n = 6) was pooled and applied to fast protein liquid chromatography analysis of cholesterol (I) and TG (J). Student's t-test was used to determine the significant differences between groups. Values of all data were mean ± SD. The significance was defined as ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001. P > 0.05, no significant difference (ns).