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. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: Biochem J. 2020 Sep 18;477(17):3433–3451. doi: 10.1042/BCJ20200546

Figure 1. Generation and validation of Arsk-deficient mice.

Figure 1.

(A) Schematic representation of the Arsk gene locus in wildtype (WT, upper panel) and Arsk knockout mice (Arsk KO, lower panel). The constitutive Arsk knockout allele (knockout first strategy) was achieved by Cre recombinase expression in B6N(Cg)-Arsktm1b(KOMP)Wtsi/J mice resulting in the loxP-dependent deletion of exon 3 of the Arsk gene as well as the neomycin phosphotransferase gene (neo) while maintaining the FRT-loxP-flanked β-galactosidase (lacZ) reporter from the targeting cassette. PCR amplicons are indicated for the WT allele (515 bp) and the knockout allele (620 bp). (B) PCR-based genotyping of mice resulted in a 620-bp- and a 515-bp-product in knockout and wildtype, respectively. NTC, no template control. (C) The absence of functional Arsk transcript was verified in various tissues of Arsk knockout mice (Arsk KO) by SYBR-green-based quantitative (q)PCR using Gapdh as reference gene.