Figure 3.
Photothermal therapy via treatment with FimH-containing thermally responsive nanoparticles (F-TRH) and NIR laser irradiation protected BALB/c mice against CT-26 carcinoma. BALB/c mice were subcutaneously injected with 1×106 CT-26-iRFP or CT-26 cells. On day 7 of the tumor challenge, the mice were intratumorally treated with PBS, FimH, HNP, TRH, and F-TRH, and irradiated with NIR laser (2 W/cm2, 5 min). (A) The images show the temperature increase after laser irradiation (left panel), and the average temperature was observed during NIR laser irradiation in CT-26 tumors (right panel), n=6, **p<0.01. (B) iRFP imaging in the tumor-bearing mice on day 10, n=5. Tumor mass in mice on day 10 (C) and day 21 (D) is shown. The data are representative of the analyses of six independent samples (ie, two samples per experiment, three independent experiments). (E) The tumor growth curve is shown, n=11 per group. (F) Survival of mice was measured, n=5 for each condition.