Fig. 6.

DDR machinery is impaired in PL-treated CRPC cells. A Cells were exposed to PL (10 μM) for 3 h and then replaced with PL-free medium for 0, 2, 4, 8, 24, and 48 h. Untreated cells served as a control. 53BP1 foci were calculated by IF using an 53BP1 antibody. Cell nuclei were stained using DAPI. Scale bar, 10 μm. B Quantification of (A). The average number of 53BP1 foci per cell was determined and ≥ 200 cells were counted each group. Values are average ± SD of at least three independent experiments. The statistical significance was calculated using the unpaired student’s two-tailed t-test with the p-values (*p < 0.05, **p < 0.01, ***p < 0.001)