Fig. 8.

Colitis increased NLRP3 inflammasome expression and accumulation of gut-derived cells in the meninges of WT mice but not NLRP3 KO mice. A Representative images of CD3 immunoexpression in the meninges (25× water immersion objective). B Representative images of CM-Dil-positive (gut-derived) cells and NLRP3 expression in the meninges (25× water immersion objective, magnified 3×). C Comparison of CD3-positive cell number (i) and CM-Dil-positive cell number (ii) in the meninges of WT and NLRP3 KO mice. D Representative images of NLRP3 inflammasome and LYVE-1 immunoexpression in the meninges (25× water immersion objective). E Comparison of NLRP3 inflammasome (i) and LYVE1 intensities (ii) in the meninges of WT and NLRP3 KO mice. Each dataset is expressed as mean ± SD. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001. n = 6 mice