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. 2021 Jul 7;21:784. doi: 10.1186/s12885-021-08344-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — MCM6 can regulate the cell cycle of neuroblastoma at both the cell and protein level. (A-D) Cell cycle analysis was performed on neuroblastoma cell lines using flow cytometry. After MCM6 silenced, SK-N-BE [2] and SK-N-SH cells both showed an increase in G1 phase components and a decrease in S phase components. (E) The expression of cell cycle regulated genes in different cell cycle phases of SK-N-BE [2] cells. (F) Verify the expression of cell cycle regulation related proteins by WB. The expression of CDK4 and CyclinD1 was down-regulated after MCM6 silence. (G-H) According to the cell cycle regulation pathway enriched by RNA-seq, the relevant genes on the pathway were verified by qPCR. The value is displayed as the mean ± SEM and statistical significance is expressed as **** p < 0.0001