Table 2.
Effects of different doses of HESS and glibenclamide on gonadal mass and sperm parameters
| Groups | TW (g) (single testis) |
GSI (%) | Total sperm count (× 107) /mLof HTF | Viability (%) | Motility (%) | DNA-damage (%) | Abnormality (%) | Maturity |
|---|---|---|---|---|---|---|---|---|
| NC | 1.62 ± 0.09b | 0.45 ± 0.05 | 99.40 ± 2.7 b | 89.4 ± 3.6 b | 87.8 ± 3.1 b | 6.4 ± 1.8 b | 11.6 ± 1.8 b | 98.4 ± 1.5 b |
| DC | 1.33 ± 0.05a | 0.55 ± 0.09 | 48.60 ± 3.5 a | 47.8 ± 5.8 a | 54.0 ± 6.0 a | 34.2 ± 2.6 a | 38.0 ± 3.4 a | 84.8 ± 0.5 a |
| HESS-100 | 1.44 ± 0.03a | 0.53 ± 0.04 | 50.40 ± 3.6 a | 58.4 ± 2.9 a | 56.6 ± 6.6 a | 30.4 ± 1.1 a | 34.0 ± 4.5 a | 86.4 ± 2.1 a |
| HESS-200 | 1.47 ± 0.07 | 0.52 ± 0.06 | 57.80 ± 4.2 a | 63.4 ± 4.0 a | 60.8 ± 4.8 a | 28.8 ± 1.6 a | 30.4 ± 5.6 a | 87.0 ± 1.2 a |
| HESS-400 | 1.52 ± 0.1b | 0.53 ± 0.01 | 60.50 ± 3.1 a | 71.0 ± 2.9 a | 63.0 ± 5.1 a | 24.7 ± 1.3 a | 28.0 ± 1.8 a | 90.2 ± 1.7 a |
| G | 1.54 ± 0.1b | 0.57 ± 0.06 | 94.20 ± 2.4b | 80.0 ± 3.8 b | 79.2 ± 4.1 b | 11.6 ± 2.4 b | 17.2 ± 2.9 b | 94.4 ± 2.7 b |
| G + HESS-200 | 1.49 ± 0.04b | 0.54 ± 0.06 | 96.80 ± 3.5 b | 85.6 ± 4.8 b | 82.4 ± 2.4 b | 11.0 ± 2.7 b | 13.0 ± 3.1 b | 95.0 ± 1.6 b |
| G + HESS-400 | 1.50 ± 0.05b | 0.52 ± 0.06 | 98.80 ± 1.9 b | 86.2 ± 4.3 b | 85.4 ± 4.6 b | 10.6 ± 2.9 b | 11.8 ± 4.1 b | 97.0 ± 2.7 b |
| P-value | 0.001 | 0.93 | 0.001 | 0.001 | 0.001 | 0.001 | 0.001 | 0.001 |
Testicular weight (TW), gonadosomatic indices (GSI), HESS (hydroalcoholic extract of S. securidaca seeds); G (glibenclamide); DC (diabetic control); NC (normal control)
a: statistically significant difference with NC group
b: statistically significant difference with DC group