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. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: J Proteome Res. 2020 Nov 11;20(1):337–345. doi: 10.1021/acs.jproteome.0c00426

Figure 1.

Figure 1.

Workflow of standard-scale and microgram-scale of TMT-LC/LC-MS/MS. (A) Standard-scale pipeline. Digested proteins (peptides) are desalted and labeled by TMT (TMT/peptide ratio of 1.5:1). The pooled samples are then desalted and analyzed by LC/LC-MS/MS. (B) Microgram-scale pipeline. Proteins in the tissue lysate are digested into peptides with excess trypsin to shorten digestion time. Without desalting, the digested tissue lysate is directly labeled by TMT (TMT/tissue ratio of 3:1). The pooled sample is analyzed by LC/LC-MS/MS with highly sensitive LC columns.