Skip to main content
. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: J Proteome Res. 2020 Nov 11;20(1):337–345. doi: 10.1021/acs.jproteome.0c00426

Figure 2.

Figure 2.

TMT reactivity of lysis buffer components and TMTpro titration. (A) Structure of TMTpro. (B) Hypothetical product of TMTpro-DTT, showing that TMT reacts with the thiol group in DTT. (C) Titration of the TMTpro zero reagent. Human brain tissue lysate (5 μg tissue containing ~0.5 μg protein) was digested and directly labeled by TMTpro zero with various TMT/tissue ratios (w/w in triplicates) for 30 min. The labeled peptides were analyzed by LC-MS/MS. The percentage of labeled peptides was defined by quantifying unlabeled peptides in each run, compared to the control run (i.e. unlabeled sample). The titration experiment was replicated (n = 3) with the standard deviation of the mean shown.