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. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: J Proteome Res. 2020 Nov 11;20(1):337–345. doi: 10.1021/acs.jproteome.0c00426

Figure 3.

Figure 3.

A flow splitter implemented in microscale HPLC. (A) Integrated scheme including a sample loading loop, HPLC pump, a reverse-phase LC column, and one flow splitter with a waste line (63.5 μm i.d. x 60 cm tubing). The pooled TMT sample was first loaded onto a 20 μl sample loop, and injected to the column at flow rate of 20 μl/min for 20 min with the splitter closed. After injection, the sample was eluted with the splitter open, which allowed for 20 μl/min column flow rate using 530 μl/min HPLC flow rate. (B) The flow rates with the flow splitter at different positions.