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. 2021 May 3;27:620256. doi: 10.3389/pore.2021.620256

FIGURE 1.

FIGURE 1

Genetic alterations of EGFR extracellular domain in PE/CA-PJ15 and PJ41 HNSCC cell lines (A) Different SNP pattern in the case of two PE/CA cell lines: PE/CA-PJ15 c720 C > T Asn158Asn homozygous SNP (upper row, left), PE/CA-PJ41 c720 C > T Asn158Asn heterozygous SNP (upper row, right), PE/CA-PJ15 c1808 G > A, Arg521Lys (middle row left); PE/CA-PJ15 c2133 T > A Thr629Thr (lower row, left). (B) investigation of EGFR vIII and EGFR wild type isoforms after mRNA isolation, reverse transcription, PCR and agarose gel electrophoresis. The left side shows that U87 vIII, an EGFR vIII overexpressing glioblastoma cell line expresses the vIII isoform of the receptor, while the two HNSCC cell lines express only the untruncated wild type isoform, showing the amplification of a large region of 896 base pairs. The right side of the image shows that wild type EGFR is present in all of the samples.