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. 2021 Mar 30;27:614281. doi: 10.3389/pore.2021.614281

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Copyright © 2021 Dastych, Hubatka, Turanek-Knotigova, Masek, Kroupa, Raška, Turanek and Prochazka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD44v8-10 overexpression in colon polyps. Position of real time primers within the CD44 mRNA transcript of CD44v8-10 (A) and CD44s (B). Reverse primers span exon junctions and anneal only if the following exon junctions occur C5+V8 (A) or C5+C15 (B). Verification of appropriate size of PCR products was performed in random samples using 3% agarose gels (C). Relative expression levels of CD44s and CD44v8-10 in colon polyps compared to adjacent healthy mucosa (D). Statistical values are calculated using one sample t test. Column mean is significantly different from hypothetical value 1.0; p = 0.018 for v8-10; p < 0.001 for CD44s number of patients N = 50.