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. 2021 Mar 5;27:581395. doi: 10.3389/pore.2021.581395

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Copyright © 2021 Kiss, Jámbor, Koroknai, Szász, Bárdos, Mokánszki, Ádány and Balázs.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Invasive potential of WM278 cells. (A) Cells were cultured in Matrigel invasion chambers, fixed and stained with hematoxylin–eosin (×100 magnification). (B) The number of invaded cells are plotted for the untreated, NC-siRNA treated and OPN-siRNA silenced cells. The data are presented as the mean ± SD of three independent experiments. Invasion was significantly lower in the silenced cells compared to the control cell lines (untreated WM278 and NC-siRNA treated WM278) (p ≤ 0.05; Student’s t-test). Asterisk indicates significant difference between the control and OPN silenced cell lines. Black column: untreated cells; gray column: Negative Control siRNA (NC-siRNA) transfected cells; white column: OPN-siRNA transfected cells).