Skip to main content
. Author manuscript; available in PMC: 2021 Jul 7.
Published in final edited form as: Stem Cells. 2014 Mar;32(3):717–729. doi: 10.1002/stem.1541

Figure 5:

Figure 5:

(A) Immunofluorescence of differentiated LFs. Differentiated LF SSEA4+ cells are positive for corneal epithelial markers CK3, CK12, CK8, CK14, CK15, β1 integrin, and E-cadherin, and negative for α-SMA. Differentiated LF SSEA4− cells are negative for corneal epithelial markers CK3, CK12, CK14, CK15 and E-cadherin, and positive for CK8, β1 integrin, and α-SMA. Scale bar 50 μm. (B) RT-PCR of LFs differentiated into corneal epithelial cells. (C) Expression of stem cell markers after differentiation in LFs. The normalized gene expression-to-GAPDH ratio is shown on the y-axis. The data represent the mean ± SEM from 6 experiments. Statistical analysis U Test (B) and Two tailed Student’s was performed using Mann-Whitney t test (C) (*p < 0.05, ** p < 0.01, *** p < 0.001). Abbreviations: LFs, limbal fibroblasts; BM MSCs, bone marrow mesenchymal stem cells; SSEA4, stage-specific embryonic antigen 4; CK, cytokeratin.