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. 2021 Apr 30;27:588084. doi: 10.3389/pore.2021.588084

FIGURE 10.

FIGURE 10

Analyses for migration ability-associated PI3K/AKT signaling pathway in LLC1 tumors of C57BL/6NKorl mice subsequent to 250, 500 and 1000GEGR. (A) Expression levels of four proteins were determined in LLC1 tumor using an imaging densitometer. (B) Expression levels of two proteins in LLC1 tumor homogenates were determined using an imaging densitometer. The level of each protein is presented relative to the intensity of actin. Two to three tumors per group were used in the preparation of the total tumor homogenate, and Western blot analysis was assayed in duplicate for each sample. (C) The levels of MMP 2, MMP 13 and Chi3L1 transcripts were measured in the total mRNA of LLC1 tumors by quantitative real time-PCR (RT-qPCR) analysis using specific primers. Two to three tumors per group were used in the preparation of the total RNAs, and RT-qPCR analysis was assayed in duplicate for each sample. Data are reported as the means ± SD. *, p < 0.05 compared to the Vehicle treated group. #, p < 0.05 compared to the Cis treated group. †, p < 0.05 compared to the 250GEGR treated group. §, p < 0.05 compared to the 500GEGR treated group.