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. 2021 Apr 30;27:588084. doi: 10.3389/pore.2021.588084

FIGURE 4.

FIGURE 4

Expression of NF-κB signaling pathway members and inflammatory cytokines. After treatment with 25, 50 and 100 μg/ml of GEGR for 24 h, total protein and RNA were collected from GEGR treated LLC1 cells. (A) Expression levels of four proteins were determined using an imaging densitometer. The level of each protein was presented relative to the intensity of actin. (B) The levels of TNF-α, IL-6 and IL-1α transcripts were detected in the total mRNA of LLC1 cells by quantitative real time-PCR (qRT-PCR) analysis using specific primers. Two to three dishes per group were used in the preparation of the total cell homogenates and RNAs, and Western blot analysis and qRT-PCR were assayed in duplicate for each sample. Data are reported as the means ± SD. *, p < 0.05 compared to the Vehicle treated group. †, p < 0.05 compared to the 25GEGR treated group. §, p < 0.05 compared to the 50GEGR treated group.