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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Photochem Photobiol. 2020 Jul 24;96(6):1314–1320. doi: 10.1111/php.13305

Figure 3.

Figure 3.

SIRT6 knockout by CRISPR/Cas9 results in alteration of key cancer-related genes. Key genes were found to be significantly modulated (>2-fold change in one clone and >1.5-fold change in the other, with statistical significance for both) after SIRT6 knockout. (A) Graphical representation of the 11 genes that were found differentially expressed upon SIRT6 KO. The data shown represent three biological replicates. (B) RT-qPCR analysis was performed to validate the key altered genes at mRNA levels in WT and SIRT6 KO cells as detailed in the Supporting Information. Data are represented as mean ± SEM of minimum three technical and two biological replicates, and were analyzed via GraphPad Prism software using multiple t-tests and the Holm-Sidak method for multiple comparisons, with significance indicated by * P≤0.05, **P<0.01, ***P<0.001, ****P<0.0001.