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. 2021 Jun 8;6(11):e139160. doi: 10.1172/jci.insight.139160

Figure 4. Silencing ADRB3 in human brown/beige adipocytes increases intracellular glycolytic flux for thermogenic activation.

Figure 4

OCR (A), quantification of basal respiration in the presence and absence of 25 mM glucose (B), and respiratory profile (C) in siRNA-Ctrl– and siRNA-ADRB3–transfected adipocytes when exposed to high glucose. (D–F) ECAR after sequential addition glucose (25 mM), oligomycin, and 2-deoxyglucose (D) and quantification of baseline ECAR (E) and glycolysis and glycolytic capacity and reserve (F) in siRNA-Ctrl and siRNA-ADRB3 adipocytes. Data are represented as mean ± SEM. Two-tailed unpaired Student’s t test and a 2-way ANOVA were used for statistical analysis. *P < 0.05; ***P < 0.001; ****P < 0.0001.