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. 2021 Jun 8;6(11):e139160. doi: 10.1172/jci.insight.139160

Figure 6. Mirabegron uncouples respiration in primary human brown/beige adipocytes, and ADRB3 is required for functional ADRB1, ADRB2 and downstream β-adrenergic signaling.

Figure 6

(A–C) OCR trace (A) and quantification of uncoupled (B) and maximal respiration (C) in siRNA-Ctrl– and siRNA-ADRB3–transfected adipocytes in the presence of 2% BSA when stimulated with 10 μM mirabegron and Fsk. (D) Time course of OCR of control transfected and siRNA-ADRB3 cells after 10 μM dobutamine, terbutaline, mirabegron, and Fsk treatment. (E and F) Quantification of uncoupled (E) and maximal respiration (F) in the presence of 2% BSA. All data presented are mean values ± SEM. Two-way ANOVA was used for statistical analysis: *when comparing basal to stimulated in siRNA-Ctrl, $when comparing basal to stimulated in siRNA-ADRB3 adipocytes, #when comparing same doses between siRNA-Ctrl and siRNA-ADRB3 adipocytes. #P < 0.05; **,$$,##P < 0.01; ***,$$$,###P < 0.001; ****,$$$$P < 0.0001.