PH and CA septic mice were treated with either anti-TIGIT mAb or the same volume of PBS (n = 7 mice/group) as described above. Mice were subjected to sham surgery as a control (n = 5 mice/group). Animals were sacrificed, and spleens were harvested 2 d after CLP. Data were derived from 2 independent experiments. (A and B) Representative flow cytometry plots of lymphocyte gating and summary data of lymphocyte numbers from PH mice. (C and D) Representative flow cytometry plots of lymphocyte gating and summary data of lymphocyte numbers from CA mice. Groups were compared with 2-way ANOVA with Tukey’s post hoc test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. PH, previously healthy; CA, cancer; CLP, cecal ligation and puncture; TIGIT, T cell Ig and ITIM domain.