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. 2021 Jun 8;6(11):e139823. doi: 10.1172/jci.insight.139823

Figure 6. Anti-TIGIT mAb resulted in decreased expression of PD-1 on T cells in CA septic mice but not PH septic mice.

Figure 6

PH and CA mice were subjected to CLP and either treated with anti-TIGIT mAb or the same volume of PBS (n = 7 mice/group) as described above. Mice were subjected to sham surgery as a control (n = 5). Animals were sacrificed, and spleens were harvested 48 h after CLP. Data were derived from 2 independent experiments. (A) Representative flow cytometry plots of PD-1 expression on T cells in sham, CLP and CLP plus αTIGIT groups with or without preexistent malignancy. (B) Data summary of PD-1 percentages on CD4+ T cells, CD8+ T cells, Treg, and Tconv cells from both PH and CA mice. Groups were compared with 2-way ANOVA with Tukey’s post hoc test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001. PH, previously healthy; CA, cancer; CLP, cecal ligation and puncture; TIGIT, T cell Ig and ITIM domain; PD-1, programmed cell death 1; Tconv, T conventional cell.