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. 2021 May 24;6(10):e143037. doi: 10.1172/jci.insight.143037

Figure 4. ZnT8B–/– hIAPP mice have increased islet amyloidogenesis, reduced β cell area, and abnormal β cell morphology.

Figure 4

(A) Thioflavin S staining (scale bar: 50 μm) with quantification of (B) amyloid severity, (C) amyloid prevalence, and (D) β cell area. (E) Transmission electron microscopy on isolated islets (animal number, n = 2–3/genotype, scale bar: 500 nm), representative cell 1 (C1), cell 2 (C2), and cell 3 (C3). (F) Quantification of the number of dense-core granules and (G) rod-like insulin granules. (H) Five-hour fasted plasma insulin, (I) 5-hour fasted plasma proinsulin, and (J) proinsulin to insulin ratio. Data are represented as mean ± SEM. **P < 0.01, ***P < 0.001, all groups compared with control, ##P < 0.01, all compared with ZnT8B–/–, $P < 0.05, $$P < 0.01, $$$P < 0.001, $$$$P < 0.0001, all groups compared with hIAPP. (A–D) Control (n = 4), ZnT8B–/– (n = 5), hIAPP (n = 6), and ZnT8B–/– hIAPP (n = 6). (H–J) Control (n = 18), ZnT8B–/– (n = 14), hIAPP (n = 17), and ZnT8B–/– hIAPP (n = 18). One-way ANOVA with Tukey’s adjustment was used in B–D and F–J.