Figure 3. The hypoxia + TGF-β1 i-T_RM transcriptional profile is enriched for endogenous human T_RM gene signatures.

CD69^–CD103^– (20% O₂), CD69⁺CD103^– (2% O₂), and CD69⁺CD103⁺ (2% O₂ + TGF-β1) CD8⁺ T cells were generated as described earlier and sorted before RNA isolation and transcriptome analysis via RNA-Seq (n = 3). (A) GSEA of relevant gene signatures derived from endogenous T_RM and resident memory-like tumor-infiltrating lymphocytes (TIL_RM) in the transcriptome of CD69⁺CD103⁺ versus CD69^–CD103^–, presented as normalized enrichment score (NES), *P adj < 0.05, **P adj < 0.01. (B) Venn diagram showing the overlap of DEGs between CD69⁺CD103⁺ and CD69^–CD103^– cells and gene sets derived from endogenous human skin (Cheuk et al., ref. 36) and lung (Hombrink et al., ref. 23) T_RM.