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. 2021 May 24;6(10):e145854. doi: 10.1172/jci.insight.145854

Figure 1. RarαDNLoxP/+; Wnt1Cre+ mice have abnormal craniofacial development.

Figure 1

(A and B) E12.5 RarαDNLoxP/+; Wnt1Cre+ (RarαDN; Wnt1Cre) mice have obvious craniofacial defects with inset eyes (black arrowhead) and midline facial cleft (white arrowhead). Scale bar: 1 mm. n = 3 control, n = 3 RarαDNLoxP/+; Wnt1Cre+. (C and D) E12.5 Alcian blue staining shows markedly reduced cartilage in neural crest–derived structures of the face. Scale bar: 1mm. n = 3 control, n = 3 RarαDNLoxP/+; Wnt1Cre+. (E and F) Representative images of H&E-stained coronal sections of RarαDNLoxP/+; Wnt1Cre+ mice show inset eyes and cleft nasal passages compared with control (Wnt1Cre+) at E12.5. Arrowhead indicates nasal cavity. Scale bar: 200 μm. n = 3 control, n = 3 RarαDNLoxP/+; Wnt1Cre+. (G–J) Dorsal root ganglia appear fairly normal at E11.5 in RarαDNLoxP/+; Wnt1Cre+; R26R-EYFP+ mice based on EYFP (G and H), HuC/D+ neuron (I and J), and TuJ1+ neurite imaging (K and L). Scale bar: 100 μm. n = 3 control, n = 3 RarαDNLoxP/+; Wnt1Cre+.