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. 2021 May 24;6(10):e145854. doi: 10.1172/jci.insight.145854

Figure 4. RAR signaling in ENCDC is required for Ret expression.

Figure 4

(A–C) Whole-mount TuJ1 and HuC/D antibody staining of E12.5 bowel from control, RarαDNLoxP/+; Wnt1Cre+, and Ret–/– mice. Note that there are no ENCDC beyond stomach in RarαDNLoxP/+; Wnt1Cre+ (B) or Ret–/– mice (C). Scale bar: 1000 μm. (D–I) IHC of E12.5 control (Wnt1Cre+; Rosa26EYFP) (D, E, and F) or RarαDNLoxP/+; Wnt1Cre+; Rosa26EYFP (G, H, and I) stomach using EYFP and RET antibodies. Scale bar: 50 μm. (J) Percentage ratio of EYFP+ ENCDC that are RET immunoreactive. Wnt1Cre+; Rosa26EYFP (n = 5 mice, 2100 cells), RarαDNLoxP/+; Wnt1Cre+; Rosa26EYFP (n = 5 mice, 1994 cells, P = 0.0002, 2-tailed unpaired Student’s t test).