Figure 6. Vagal nerve fibers occupy a smaller area in E11.5 stomach of RarαDN^LoxP/+; Wnt1Cre⁺ mice than in controls.

(A and B) TuJ1 antibody–stained E11.5 stomach of RarαDN^LoxP/+ (control) (A) or RarαDN^LoxP/+; Wnt1Cre⁺ (B) mice. Scale bar: 500 μm. (C) Quantification of TuJ1⁺ stained vagal fiber area. **P < 0.01 by 2 tailed unpaired Student’s t tests. (D) Whole embryo imaging of E10.5 Wnt1Cre⁺; R26R-TdTomato⁺ and RarαDN^LoxP/+; Wnt1Cre⁺; R26R-TdTomato⁺. Scale bars: 500 μm (left) and 200 μm (right, enlarged). Note that TuJ1⁺ vagal nerve fibers are not TdTomato⁺. Box shows region of the magnified image. n = 3, Wnt1Cre⁺; R26R-TdTomato⁺. n = 3, RarαDN^LoxP/+; Wnt1Cre⁺; R26R-TdTomato⁺. Supplemental Video 1 and Supplemental Video 2 show 3-dimensional Z-stacks from embryos in D.