Fig. 1.

N gene detection designs and characterization. (a) Improved and new primers-probe sets design along SARS-CoV-2 N gene. Numbers in red indicate bp region amplified in RT-qPCR with the relevant primers-probe set. (b) List of Improved and new primers-probe sets sequences. (c) Calibration curves for all examined primers-probe sets for N gene detection. Resulted Ct value from RT-qPCR plotted against Log copy number tested. Error bars present standard deviation for six replicates. (d) List of Limit of detection, Linear regression R², and Y intercept values extracted from the resulted calibration curves. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)