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. 2021 Jun 22;6(12):e148035. doi: 10.1172/jci.insight.148035

Figure 1. Schematic demonstration of study workflow.

Figure 1

EM and uninvolved skin samples were first isolated from patients by punch biopsy and submitted for scRNA-Seq. In cohort 1 (n = 6), paired PBMCs were prepared as unpaired bulk TCRB and IGH repertoires from bulk RNA and also submitted for repertoire sequencing using the 10× Genomics Chromium platform. The characteristics of B cells and T cells found in both compartments were analyzed based on gene expression and features of their respective V(D)J sequences. Cohort 2 (n = 4) subject samples were sequenced using a nanowell-based single-cell transcriptome approach (Seq-Well) to support findings from cohort 1. EM, erythema migrans; sc, single-cell.