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. 2021 Jun 22;6(12):e148035. doi: 10.1172/jci.insight.148035

Figure 3. B cells in EM lesions express high levels of MHC class II genes and signatures of IFN gene signaling and antigen presentation.

Figure 3

(A) Heatmap of top 20 and bottom 20 DEGs for B cells from EM compared with B cells from uninvolved skin based on absolute average minimum gene expression difference (see Methods). (B) enrichR gene ontology analysis of the top 40 genes upregulated in B cells from EM tissue. Red bars correspond to the top significantly associated gene ontology assignments (P < 0.05 by Wilcoxon’s test). Gene expression of the “HLA-DRA” MHCII gene displayed over a UMAP projection of all single-cell gene expression data from the 6 subjects with the intensity of shading correlated with the normalized expression in either uninvolved (C) or EM skin (D). The arrow points to the location of the B cell single-gene expression cluster. Data for the same n = 6 subjects from cohort 1 are shown for all panels. EM, erythema migrans; DEGs, differentially expressed genes.